Diesel exhaust particles increase NF-kappa B DNA binding activity and C-FOS proto-oncogene expression in human bronchial epithelial cells

There is increasing evidence that diesel exhaust particles (DEP) could be i ncriminated in respiratory diseases. They have been shown to induce an infl ammatorq response in the lung and are suspected to be carcinogenic because of the presence of polyaromatic hydrocarbons (PAH) on their surface. DEP we re tested on a human bronchial epithelial cell line (16HBE) in comparison w ith carbon black particles (CB) devoid of PAH. DEP and CB at 10 mu g/cm(2) induced the release of the lactate dehydrogenase (LDH) by 16HBE cells from 48 hr of exposure. DEP at 5 mu g/cm(2) but not CB activated the binding of the nuclear factor kappa B (NF-kappa B) to DNA from 2 hr of exposure up to 15 hr. NF-kappa B is a transcription factor involved in the expression of s ome cytokines such as IL-8 and GM-CSF which have been shown to be released by 16HBE cells after DEP exposure. In addition, DEP as well as CB induced t he expression of the c-fos proto-oncogene. Taken together, these neu data s uggest that the activation of NF-kappa B and the expression of c-fos could contribute to the proliferation and chronic inflammation processes induced in lungs after DEP exposure. (C) 1999 Elsevier Science Ltd. All rights rese rved.