THE AFFINITY TO THE BRAIN DOPAMINE D-1 RECEPTOR IN-VITRO OF TRIPRENYLPHENOLS ISOLATED FROM THE FRUIT BODIES OF ALBATRELLUS-OVINUS

Six triprenyl phenols that inhibit the in vitro binding of H-3-SCH 233 90 to the dopamine D-1 receptor subfamily in rat striatal brain membra nes were isolated from extracts of the edible mushroom Albatrellus ovi nus. The compounds, of which scutigeral 1a, ilicicolin B 3, neogrifoli n 5a and grifolin 6 are known while ovinal 2 and ovinol 4a are new, we re isolated by chromatography and their structures were determined by spectroscopic techniques The IC50 values of the most potent compound, scutigeral 2a, is 2.6 mu M and it was shown that it decreases the bind ing affinity of H-3-SCH 23390 in a competitive manner. Compounds 1-5 s howed no inhibition on the binding of H-3-spiperone to the dopamine D- 2 receptor subfamily, indicating that these compounds interact selecti vely with the dopamine D-1 receptor subfamily.