M. Lovren et al., Gonadotrophs in long-term ovariectomized rat females treated with luteinizing hormone releasing hormone and estradiol, ACT VET BEO, 49(2-3), 1999, pp. 117-126
The effects of multiple estradiol dipropionate (EDP) treatment, high doses
of luteinizing hormone releasing hormone (LHRH) and the combination of both
on immunohistochemical and morphometric parameters of gonadotrophic cells
in long-term ovariectomized (ovx) rats were studied using rabbit anti-rat b
eta-follicle stimulating hormone beta-FSH) and beta-luteinizing hormone (be
ta-LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical proce
dure. A morphometric method for determining changes of gonadotrophic cell v
olume, as well as of their number and relative volume density was used. All
examined morphometric values in treated females showed a significant decre
ase in comparison with the ovx controls, the most significant decrease bein
g observed in EDP and EDP+LHRH-treated rats. These results indicate that be
sides inhibitory action of LHRH and a singificant inhibitory effect of EDF:
the combination of both expressed a prominent inhibitory effect on the exa
mined parameters of gonadotrophic cells in long-term ovx females.