FUSION EXPRESSION OF MUTATED CECROPIN CMIV IN ESCHERICHIA-COLI

Authors
XIE W QUI QF DONG XY HUA ZC XU XX
Citation
W. Xie et al., FUSION EXPRESSION OF MUTATED CECROPIN CMIV IN ESCHERICHIA-COLI, SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 40(3), 1997, pp. 225-231
Citations number
15
Categorie Soggetti
Biology
Journal title
SCIENCE IN CHINA SERIES C-LIFE SCIENCES → ACNP
ISSN journal
10069305
Volume
40
Issue
3
Year of publication
1997
Pages
225 - 231
Database
ISI
SICI code
1006-9305(1997)40:3<225:FEOMCC>2.0.ZU;2-Q
Abstract
A cDNA coding mutated cecropin CMIV from Bombyx mori was synthesized a ccording to its amino acid sequence using E. coil biased codons. The g ene was cloned into the fusion expression vector pEZZ318 and was expre ssed in E. coli HB101. The fusion protein produced was purified by aff inity chromatography to yield 26 mg/L fusion product. The anti-bacteri al activities of recombinant cecropin CMIV were recovered after cleava ge by chemical method.