Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway

Citation
H. Kobuchi et al., Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway, AM J P-CELL, 46(3), 1999, pp. C403-C411
Citations number
59
Categorie Soggetti
Cell & Developmental Biology
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
ISSN journal
03636143 → ACNP
Volume
46
Issue
3
Year of publication
1999
Pages
C403 - C411
Database
ISI
SICI code
0363-6143(199909)46:3<C403:QIIIEI>2.0.ZU;2-#
Abstract
Quercetin inhibits inducible ICAM-1 expression in human endothelial cells t hrough the JNK pathway. Am. J. Physiol. 277 (Cell Physiol. 46): C403-C411, 1999.-The cell adhesion molecule intercellular adhesion molecule-1 (ICAM-1) plays a pivotal role in inflammatory responses. Quercetin (3,3',4',5,7-pen tahydroxyflavone), a naturally occurring dietary flavonol, has potent anti- inflammatory properties. The effect of quercetin on ICAM-1 expression induc ed by agonists phorbol 12-myristate 13-acetate (PMA) and tumor necrosis fac tor-alpha (TNF-alpha) in human endothelial cell line ECV304 (ECV) was inves tigated. Quercetin treatment downregulated both PMA- and TNF-alpha-induced surface expression, as well as the ICAM-1 mRNA levels, in ECV cells in a do se-dependent (10-50 l-IM) manner. Quercetin had no effect on PMA- or TNF-al pha-induced nuclear factor-KB (NF-KB) activation. However, under similar co nditions a remarkable dose-dependent downregulation of activator protein-1 (AP-1) activation was observed. This decrease in AP-1 activation was observ ed to be associated with the inhibitory effects of quercetin on the c-Jun N H2-terminal kinase (JNK) pathway. These results suggest that quercetin down regulates both PMA- and TNF-alpha-induced ICAM-1 expression via inhibiting both AP-1 activation and the JNK pathway.