In vitro models to study mechanisms involved in cyclosporine A-mediated glomerular contraction

The immunosuppressive drug, cyclosporin A (CsA), which is successfully used to prevent rejection in organ transplantation, induces renal side-effects as shown by a decrease in glomerular filtration rate and ultrafiltration co efficient regulated by the tone of mesangial cells. The aim of the present study was to investigate the effect of CsA on isolat ed glomeruli and mesangial cells, which constitute appropriate in vitro mod els for renal vasoreactivity studies. The roles of different intracellular and extracellular mediators such as calcium, endothelin-1 (ET-1), prostagla ndins (TXA(2) and PGI(2)) and reactive oxygen intermediates (ROIs) were ana lysed. CsA caused a concentration- and time-dependent decrease in the planar cross -sectional areas of isolated glomeruli and mesangial cells as determined by image analysis. Intracytosolic free calcium concentration determined by fl uorimetric analysis was significantly increased after 30 min CsA (10 mu M) incubation. In the contraction experiment, the calcium antagonist verapamil inhibited the CsA response. ET-1, TXB2 and keto-PGF(1 alpha) were determin ed directly, however no changes were found statistically significantly diff erent from respective controls. In contrast to these results, the ET-1 spec ific antibody was able to reduce CsA-mediated cell contraction. In the pres ence of a prostacyclin agonist iloprost, CsA-induced contraction was also m odified. The role of ROIs using a 2'7'-dichlorofluorescein diacetate (DCFdA c) fluorimetric method was directly determined by observing, with 10 mu M C sA, a significant production of hydrogen peroxide (H2O2), which was able al one to induce mesangial cell contraction. Coincubation with the antioxidant s led to a significant inhibition of mesangial cell contraction. These resu lts suggest that CsA caused an imbalance in the normal level of all investi gated vasoconstrictive and vasodilator mediators, which shifted towards the advantage of vasoconstrictive action.