Nitric oxide synthase imunolabeling in the molluscan CNS and peripheral tissues

Citation
Wj. Hurst et al., Nitric oxide synthase imunolabeling in the molluscan CNS and peripheral tissues, BIOC BIOP R, 262(2), 1999, pp. 545-548
Citations number
22
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
262
Issue
2
Year of publication
1999
Pages
545 - 548
Database
ISI
SICI code
0006-291X(19990827)262:2<545:NOSIIT>2.0.ZU;2-G
Abstract
NOS immunoreactivity was assayed in CNS and peripheral tissues of the sea s lugs Pleurobranchaea californica, Tritonia diomedea and Aplysia californica using different antisera against mammalian nitric oxide synthase in Wester n blots. Polyclonal anti-nNOS labeled at 250, 185, 170, 155, 100, 75, and 6 5 kD in extracts of Pleurobranchaea CNS, salivary gland and esophagus but n ot of gills or muscle. The labeling pattern for Tritonia in bands at 250, 2 00, 120/110, 100, 69, 65, and 60 kD differed somewhat. Anti-nNOS labeling i n Aplysia was markedly different, with bands labeled only at 69 and 60 kD i n CNS extracts, and at 200, 190, 69 and 60 kD in salivary and esophagus ext racts. The wide variation in NOS immunoreactivity is consistent with specie s differences in tissue localization and biochemical properties of mollusca n NOS isoforms. (C) 1999 Academic Press.