Molecular cloning of a cold-shock domain protein, zfY1, in zebrafish embryo

Cold-shock domain proteins in vertebrates contain a highly conserved domain which is related to the Escherichia coli cold-shock proteins. Here we repo rt the cloning of a cold-shock domain protein from zebrafish embryo. Using the combination of PCR techniques with degenerate primers, 5'RACE and 3'RAC E, the full length cDNA of a cold-shock domain protein in the zebrafish emb ryo was successfully cloned without constructing and screening a library. D etermined from the deduced amino acid sequence, this protein is most simila r to Xenopus, FRGY1, and this newly cloned zebrafish gene was therefore des ignated as zfY1. (C) 1999 Elsevier Science B.V. All rights reserved.