INHIBITORY ACTIVITY OF 3'-FLUORO-2' DEOXYTHYMIDINE AND RELATED NUCLEOSIDE ANALOGS AGAINST ADENOVIRUSES IN-VITRO

Antiviral effects of nucleoside analogues against human adenoviruses ( ADV) belonging to subgroup B (ADV3) and C (ADV2) were comparatively an alysed using focus reduction assay on Fogh and Lund (FL) cells. 3'-Flu oro-2'-deoxythymidine (FTdR), 3'-fluoro-2'-deoxyuridine (FUdR), 2',3'- dideoxycytidine (ddC) and 3'-fluoro-2'-deoxyguanosine (FGdR) emerged a s potent and selective inhibitors. They were nontoxic for the FL cells at the tested doses. FTdR was proved to be the most effective inhibit or against both serotypes ADV2 and ADV3 (0.05 mu M/0.02 mu M). The inh ibitory effect of FTdR was also analyzed on the level of viral protein s and viral DNA synthesis using radioimmunoprecipitation and PCR, resp ectively. Neither the main structural protein of ADV, the hexon, nor v iral DNA. could be detected in ADV-infected FL cells that had been exp osed to FTdR. (C) 1997 Elsevier Science B.V.