New techniques in diagnostics by capillary electrophoresis of hereditary disorders of purine and pyrimidine metabolism

Purine and pyrimidine compounds were analyzed by capillary electrophoresis in urine of patients with inherited disorders purine and pyrimidine metabol ism. Two separation of systems (alkaline-anionic and acid-cationic mode) ha ve been compared. The use of a strongly acidic background electrolyte (pH 1 .8) improves the selectivity and informative pithiness of the method. A com bination of both anionic and cationic modes increases the reliability of di agnosis in hereditary disorders.