Visual drop test of hemoglobin in whole blood by using ring-like concentration of rhodamine B onto octadecylsilanized silica plate

It has been found that some dye solutes become concentrated along the perim eter of an aqueous drop sitting on an octadecylsilanized silica plate. The fluorescent ring of Rhodamine B was applied to a visual test of hemoglobin in whole blood. When a drop of diluted blood and the dye is set on the plat e, the resulting fluorescent ring can be observed through the drop with an ultraviolet lamp in the dark only for the samples of low hemoglobin content .