Newt RAD51: Cloning of cDNA and analysis of gene expression during spermatogenesis

A cDNA encoding a newt homolog of Escherichia coli RecA and yeast RAD51 fro m a testis cDNA library was isolated. The newt RAD51 (nRAD51) cDNA predicte d a 337 amino acid protein with a 95-96% amino acid identity to Xenopus and mammalian RAD51. Northern blot analysis showed that nRAD51 mRNA, 1.7 kb in length, was expressed strongly in the testis and ovary, but weakly in the liver, kidney and brain. in situ hybridization revealed that expression of nRAD51 mRNA was barely observed in primary spermatogonia (one cell in a cys t) and early secondary spermatogonia (two to four cells in a cyst), but inc reased in late secondary spermatogonia (greater than or equal to eight cell s in a cyst), reaching a maximum level in leptotene-zygotene spermatocytes, and thereafter declined. These results suggest that nRAD51 is involved in mitotic recombination in spermatogonia as well as in meiotic recombination in spermatocytes.