A maternal RNA encoding smad1/5 is segregated to animal blastomeres duringascidian development

Expressed Sequence Tag (EST) research on the ascidian Halocynthia roretzi r evealed that Hrsmad1/5, a homolog of smad genes, is expressed in H, roretzi eggs. A comparison of amino acid sequences of smad family members showed t hat the isolated clone was a homolog of smad1 and smad5 of vertebrates. A m olecular phylogenetic analysis showed that Hrsmad1/5 was separated from the common ancestor with the group containing smad1 and smad5. A northern blot analysis showed that transcript of Hrsmad1/5 was abundant in the fertilize d egg. The amount of the transcript remained constant until the gastrulae a nd then rapidly decreased at the neurulae. The spatial expression of Hrsmad 1/5 was investigated by means of whole-mount in situ hybridization. Materna l transcripts of Hrsmad1/5 were detected in the entire fertilized egg. The signals were localized preferentially to the animal blastomeres of the 8-, 16-, 32- and 64-cell stages. The zygotic expression of Hrsmad1/5 started in prospective epidermal blastomeres in the animal hemisphere at the 64-cell stage but not in cells of the central nervous system, and it decreased rapi dly around the neural-plate stage. At the tail-bud stage, signals were dete cted broadly all through the trunk and in a small part of the epidermis in the tail region. This is the first report of a maternal RNA that preferenti ally accumulates in the animal hemisphere in early ascidian embryos. Animal blastomeres of ascidian embryos differentiate mainly into epidermis in a c ell-autonomous manner and partly differentiate into neural tissues by induc tion. The Hrsmad1/5 gene may play a role in the signal transduction process in epidermal precursor cells of ascidian embryos.