THE WELL-BALANCED NUCLEOSIDE-NUCLEOTIDE MIXTURE OG-VI FOR SPECIAL MEDICAL PURPOSES

Nucleotides and nucleosides are essential components in all cells. How ever, nucleotides have not been supplied in parenteral nutrition regim ens. We developed a well-balanced nucleoside-nucleotide mixture ''OG-V I'' and examined its effect in animals under some stressed conditions. OG-VI was composed of 30 mmol/l of inosine, 30 mmol/l of guanosine mo nophosphate, 30 mmol/l of cytidine, 22.5 mmol/l of uridine and 7.4 mmo l/l of thymidine. The whole body protein turnover increased significan tly in rats receiving total parenteral nutrition (TPN) with OG-VI solu tion after 70% hepatectomy, compared with rats receiving normal TPN wi thout OG-VI (122.1 +/- 20.9 mgN.kg(-1).h(-1) vs. 97.4 +/- 10.1 mgN.kg( -1).h(-1), P < 0.01, n = 10). OG-VI significantly enhanced protein syn thesis while it did not decrease protein breakdown. The effect of OG-V I on myocardium after hypoxic challenge was also examined in rats. The creatine phosphate (PCr)/inorganic phosphate (Pi) was decreased in no rmal rat myocardium after hypoxic challenge. However, in the rats admi nistered OG-VI, PCr/Pi was maintained at baseline level and did not de crease after hypoxia. There was no significant change in the level of adenosine triphosphate (ATP) between before and after hypoxic challeng e in myocardium of the rats administered OG-VI. in the rats receiving normal saline, instead of OG-VI, the ATP level decreased significantly after hypoxic challenge (4132 +/- 276 nmol/g tissue, n = 3, vs. 3439 +/- 465 nmol/g tissue, n = 5, P < 0.05). These data suggested that the well-balanced nucleoside-nucleotide mixture, OG-VI improved nitrogen metabolism and might stimulate synthesis of high-energy phosphate in r ecovery after severe surgical stress. (C)Elsevier Science Inc. 1997.