The complete cDNA sequence encoding dog gastric lipase

Oligodeoxyribonucleotide ligation to single-stranded cDNA (SLIC) and polyme rase chain reaction (PCR) techniques were used to clone an entire dog gastr ic lipase (DGL) cDNA. The size of the cDNA is confirmed by Northern blot an alysis. The DGL is synthesized as a 379-amino acid mature polypeptide with a molecular mass of 43176 Da which is preceded by a 19-amino acid signal se quence located at the NH2-terminus. Comparison of the signal sequences reve als a high degree of similitude between the DGL, the human gastric lipase ( HGL), the rabbit gastric lipase (RGL) and the rat lingual lipase (RLL).