Q. Ren et al., Glucuronidation of the lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) by rat UDP-glucuronosyltransferase 2B1, DRUG META D, 27(9), 1999, pp. 1010-1016
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone and its major metabolite, 4-
(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), are potent lung carcino
gens in animals. UDP-glucuronosyltransferase (UGT)-mediated glucuronidation
of NNAL is a potentially important detoxification pathway for these carcin
ogens. To identify the UGT isozyme(s) involved in this pathway, we examined
the glucuronidation of NNAL in rat liver microsomes and homogenates from c
ell lines overexpressing specific UGT isozymes. NNAL glucuronidation was in
duced in liver microsomes from rats treated with family 2 UGT inducers incl
uding phenobarbitol and 3,5-di-tert-butyl-4-hydroxytoluene, which exhibited
1.7- and 2.6-fold higher rates of glucuronidation than microsomes from con
trol rats. The rates of NNAL glucuronidation in liver microsomes from GUNN
(deficient in family 1 UGTs) and RHA parental control rats were similar. Al
l rat liver microsomes used in the present study catalyzed the glucuronidat
ion of (S)-NNAL at a rate between 3.5 and 5.5 times that of the glucuronida
tion of (R)-NNAL. Liver microsomes from Wistar rats exhibiting the low-andr
osterone glucuronidation phenotype characteristic of the UGT2B2-deficient g
enotype glucuronidated NNAL at a rate similar to microsomes from Wistar rat
s exhibiting the high-androsterone glucuronidation phenotype/UGT2B2 [+] gen
otype. Homogenates from UGT2B1-overexpressing cells catalyzed the glucuroni
dation of NNAL at a K-m of 745 mu M. As with rat liver microsomes, NNAL-GIu
c I was the major diastereomer formed by UGT2B1. Glucuronidation of NNAL wa
s not detected with homogenates from UGT2B12-overexpressing cells. These re
sults suggest that UGT2B1 plays an important role in the glucuronidation of
NNAL in the rat.