Respiratory tract inflammation during the induction of chronic bronchitis in rats: role of C-fibres

The hypothesis that chronic stimulation of C-fibres by inhaled irritants co ntributes to the inflammatory changes that occur during the development of chronic bronchitis was tested. The effect of neonatal capsaicin pretreatment on the development of respira tory tract inflammation was examined in a rat model of chronic bronchitis i nduced by SO2 exposure. Adult capsaicin- and vehicle-treated rats were expo sed to SO2 (250 parts per million (ppm) 5 h.day(-1)) for one day, 2 weeks o r 4 weeks. Nasal (NL), airway (AL) and bronchoalveolar (BAL) lavages were p erformed and the number and types of cells in the lavage fluids measured. S O(2)induced changes in ventilation were also measured on day 1 of SO2 expos ure and in the 3rd and 5th week of exposure. In the vehicle-treated rats, neutrophils became elevated in NL after just o ne day of SO2 exposure, in AL after 2 weeks, and in the BAL after 4 weeks. In comparison to vehicle animals, more neutrophils were recovered in the AL of capsaicin-treated rats after one day of SO2 (p=0.012), and in the BAL a fter 2 or 4 weeks (p=0.004 and p=0.01, respectively). On day 1, SO2 caused a transient increase in tidal volume and a sustained decrease in frequency that was not different in capsaicin- and vehicle-treated rats. With continu ed exposure, these ventilatory responses to SO2 were attenuated in both gro ups of rats. These data support the hypothesis that the presence of C-fibres limits or d elays the inflammation that occurs during the development of chronic bronch itis induced by SO2 exposure. The protective effect of C-fibres is not the result of ventilatory responses to stimulation of these afferents.