Bromine K-edge EXAFS studies of bromide binding to bromoperoxidase from Ascophyllum nodosum

Authors
Dau, H Dittmer, J Epple, M Hanss, J Kiss, E Rehder, D Schulzke, C Vilter, H
Citation
H. Dau et al., Bromine K-edge EXAFS studies of bromide binding to bromoperoxidase from Ascophyllum nodosum, FEBS LETTER, 457(2), 1999, pp. 237-240
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
457
Issue
2
Year of publication
1999
Pages
237 - 240
Database
ISI
SICI code
0014-5793(19990827)457:2<237:BKESOB>2.0.ZU;2-8
Abstract
Bromine K-edge EXAFS studies have been carried out for bromide/peroxidase s amples in Tris buffer at pH 8, The results are compared with those of aqueo us (Tris-buffered) bromide and vanadium model compounds containing Br-V, Br -C(aliphatic) and Br-C(aromatic) bonds. It is found that bromide does not c oordinate to the vanadium centre. Rather, bromine binds covalently to carbo n. A possible candidate is active site serine, (C) 1999 Federation of Europ ean Biochemical Societies.