Cytogenetic abnormalities involving the 11q23 region are found in both acut
e lymphoblastic leukemia (ALL) and myeloid leukemia (AML). Molecular conseq
uences of 11q23 translocations are the formation of chimeric genes, all of
them involving the MLL (mixed-lineage leukemia) gene. To evaluate the usefu
lness of fluorescence in situ hybridization (FISH) in detecting MLL rearran
gements in AML, we analyzed 181 patients with an MLL-specific probe. Among
them, we detected three patients with multiple FISH signals, reflecting gen
omic amplification of this chromosomal region. Extra copies of MLL have bee
n reported previously in four patients, but did not correspond to a true ge
ne amplification. For the first time, we describe genomic amplification of
the 11q23 region (up to more than 50 copies) in AML patients. This genomic
amplification could affect MLL, but other genes in the vicinity could also
be the primary target. Genes Chromosomes Cancer 26:166-170, 1999. (C) 1999
Wiley-Liss, Inc.