M. Xu et al., Thermal radiosensitization of human tumour cell lines with different sensitivities to 41.1 degrees C, INT J HYPER, 15(4), 1999, pp. 279-290
While much work on radiosensitization by hyperthermia in the 43 degrees C a
nd higher temperature range has been done, relatively little work has been
done at temperatures in the 41-42 degrees C range. In this moderate hyperth
ermia range there are dramatic differences in the resistance of mammalian c
ells to hyperthermia. Therefore, thermal radiosensitization was measured in
two human colon adenocarcinoma cell lines, one that expresses chronic ther
motolerance and proliferates at 41.1 degrees C, NSY 42129 (NSY) cells and o
ne that is slowly killed at 41.1 degrees C, HCT15 cells. Heat-resistant NSY
cells were found to be more radioresistant than heat-sensitive HCT15 cells
. Hyperthermia at 41.1 degrees C enhanced the radiation sensitivity in NSY
cells, but no significant induction of heat-induced radiosensitization was
observed in HCT15 cells. The radiation sensitivity induced by 41.1 degrees
C in NSY cells appeared to be related to both intrinsic heat-induced radios
ensitization (HIR) and cell-cycle redistribution at 41.1 degrees C. Inciden
tally, cells incubated at 41.1 degrees C for between 8-16 h displayed an id
entical radiosensitivity to those heated for 24 h. This result implies that
modest hyperthermia for 2 h or more can have a radiosensitizing effect in
heat-resistant cells.