Effects of hydrosalpingeal fluid on murine embryo development and implantation

Purpose: Our purpose was to evaluate the effects of various concentrations of hydrosalpingeal fluid (HSF) on the preimplantation development and impla ntation of murine embryos. Methods: One-cell mouse embryos were cultured in KSOM culture medium with 0 .1, 1.0, 10, or 50% HSF: without and with lactate supplementation. Late-sta ge embryos were transferred into the uteri of pseudopregnant CD-1 females t o determine implantation rates. The embryo transfer technique used was deve loped by our group and its effectiveness was evaluated during this experime nt. Results: Blastocyst development in the 0.1, 1.0, 10, and 50% group was 45 5 5.0 12.5, and 17.5%, respectively, with lactate supplementation, and 35.0 5 2.5, 12.5, and 5.0%, respectively, without lactate supplementation, while i n the KSOM (control) group it was 63. 8%. Blastocyst development was reduce d compared to controls in the 10% HSF and 50% HSF groups. Implantation rate s for the 0.1 and 1.0% groups with lactate supplementation were 43.0 and 25 .0%, respectively, and those with lactate supplementation were 50.6 and 61. 8%, respectively, while in the KSOM group the implantation rare was 65.5%. None of the implantation rates were significantly different. Conclusions: Hydrosalpingeal fluid has a concentration-dependent inhibitory effect on in vitro murine embryo development, but it has minimal effects o n implantation rates.