A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin

A pair of degenerate PCR primers (FAP59/64) was designed from two relativel y conserved regions of the L1 open reading frame of most human papillomavir uses (HPV), The size of the generated amplicon was about 480 bp, PCR using these primers was found capable of amplifying DNA from 87% (65/75) of the H PV types tested, its sensitivity being 1-10 copies for HPV-5, -20 and -30 c lones, HPV was found in 63% (5/8) of tumour samples and in 63% (5/8) of nor mal skin biopsies from patients with various cutaneous tumours, HPV-5, HPV- 8, HPV-12, HPVvs20-4 and six putatively novel HPV types were identified, No correlation was found to exist between specific HPV and tumour types, Skin surface swab samples from one or more sites on three of four healthy volun teers were found to contain HPV, types 12 and 49 being identified, as well as eight novel HPV types, two of which were also found among the patients, In all, HPV was detected in 75% (9/12) of those tested, five HPV types and 12 novel candidate types being identified, and 37% (7/19) of HPV-positive s amples were found to manifest more than one HPV type, All the HPV detected manifested high degrees of nucleotide sequence similarity with HPV types as sociated with skin lesions and epidermodysplasia verruciformis, The overall HPV finding in the skin samples was 50% (20/40) using the FAP primers as c ompared to 18% (7/40) using another PCR test designed for skin types, The r esults thus suggest the new method to be sensitive and generally applicable for detecting cutaneous HPV.