Reversed-phase ion-pair thin layer chromatography of some alkaloids

The retention behaviour of some alkaloids, as test samples, on reversed-pha se pre-coated thin layer chromatography in the presence of different ion-pa ir reagents was investigated. Trifluoroacetic acid (TFA), pentafluoropropio nic acid (PFPA), and heptaflluorobutyric acid (HFBA) were used as counter i ons. The dependence of R-F, values on the eluent composition, the chain len gth of the ion-pair reagent and the ratio of two ion-pair reagents in the e luent is described. The retention values of the tested alkaloids after impr egnation of the plate with the counter ion, were investigated.