Cloning of a full length cDNA encoding canine interleukin-4

Primer designed on the basis of the conserved transcription start point (ts p) region of human and bovine gene transcripts, encoding the Interleukin-4 (IL-4), and subsequently the gene specific primer and an adaptor primer pai r, was successfully used, to generate the full-length complementary DNA (cD NA) sequence coding for canine IL-4 (cIL-4), from pokeweed mitogen stimulat ed canine peripheral blood lymphocytes. The full-length cIL-4 contains an o pen reading frame of 399 nucleotides (nt), with a 5' ends of 66 base pairs (bp) and 3' ends of 125 bp. The nucleotide sequence contains six possible A sn-X-Thr or Asn-X-Ser linked glycosylation sites. Five sequence motifs of T ATT or ATTTA, responsible for the regulation of gene expression, are found in the 3' untranslated region.