Preparation of highly purified momordin II without ribonuclease activity

Momordin II, a ribosome-inactivating protein from Momordica charantia seeds , was purified by a procedure involving a series of chromatographies on S-S epharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly pu rified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.