The influence of ethanol and magnesium on aminoacyl-tRNA synthetases activity in rat's liver

Ethanol exerts various influences on living organisms. Experiments were con ducted in order to determine the influence of ethanol and magnesium on the aminoacyl-tRNA synthetases (aaRS) activity in rat's liver. The experiment w as conducted on white Wistar rats of both sexes. The animals were divided i nto groups of 10 rats each. They received ethanol in their drinking water f or 6 weeks. The first group received 5 per cent ethanol, the second receive d 20 per cent ethanol, the third received 20 per cent ethanol and 200 ppm m agnesium, the fourth was the control and received water. After the duration of the experiments the animals were sacrificed using ketamine and the live rs were harvested for further testing. Aminoacyl-tRNA synthetases were obta ined by salting out with ammonium sulphate. The aminoacyl-tRNA synthetases activity were tested in vitro by using marked amino acids. Ethanol influenc es aminoacyl-tRNA synthetases activity. The influence of ethanol on the activity of aaRS depended on the size of th e dose of ethanol taken by examined animals. At the same time it was reveal ed that magnesium supplementation limits the negative effect of high ethano l doses.