NUCB1, the Drosophila melanogaster homolog of the mammalian EF-hand proteins NEFA and nucleobindin

Citation
S. Otte et al., NUCB1, the Drosophila melanogaster homolog of the mammalian EF-hand proteins NEFA and nucleobindin, MECH DEVEL, 86(1-2), 1999, pp. 155-158
Citations number
14
Categorie Soggetti
Cell & Developmental Biology
Journal title
MECHANISMS OF DEVELOPMENT
ISSN journal
09254773 → ACNP
Volume
86
Issue
1-2
Year of publication
1999
Pages
155 - 158
Database
ISI
SICI code
0925-4773(199908)86:1-2<155:NTDMHO>2.0.ZU;2-5
Abstract
Mammalian NEFA and nucleobindin are calcium-binding proteins containing a s ignal peptide, two EF-hand motifs, acidic and basic regions and a leucine-z ipper motif. Although they have been discussed to be involved in autoimmuni ty, apoptosis and calcium homeostasis in the Golgi apparatus and bone matri x, their exact role remains unknown. Here we report the cloning of their Dr osophila homolog, nucb1, as well as the analysis of its expression pattern during embryogenesis and the subcellular localization of the NUCB1 protein. The nucb1 mRNA and the NUCB1 protein were found to be expressed maternally and zygotically, and they accumulate ubiquitously at low levels during all embryonic stages due to a maternal component. From stage 11 onward, high l evels of zygotic expression can be detected specifically in the salivary gl ands and their placodes. In contrast to the known mammalian family members, the NUCB1 protein localizes in a subpattern of cytoplasmic substructures, probably the Golgi apparatus. (C) 1999 Elsevier Science Ireland Ltd. All ri ghts reserved.