Fractal-based image analysis methods are investigated to extract textural f
eatures related to the anisotropic structure of trabecular bone from the X-
ray images of cubic bone specimens. Three methods are used to quantify imag
e textural features: power spectrum, Minkowski dimension and mean intercept
length. The global fractal dimension is used to describe the overall rough
ness of the image texture. The anisotropic features formed by the trabecula
e are characterised by a fabric ellipse, whose orientation and eccentricity
reflect the textural anisotropy of the image. Tests of these methods with
synthetic images of known fractal dimension show that the Minkowski dimensi
on provides a more accurate and consistent estimation of global fractal dim
ension. Tests on bone x-ray (eccentricity range 0.25-0.80) images indicate
that the Minkowski dimension is more sensitive to the changes in textural o
rientation. The results suggest that the Minkowski dimension is a better me
asure for characterising trabecular bone anisotropy in the x-ray images of
thick specimens.