Allo-immunization elicits CD8(+) T cell-derived chemokines, HIV suppressorfactors and resistance to HIV infection in women

We assessed the potential for an allogeneic-based vaccine against HIV infec tion in women who were allo-immunized with their partners' mononuclear leuc ocytes to prevent spontaneous recurrent abortion. Within 1 month of allo-im munization, there was significant upregulation in the concentrations of CD8 cell-derived suppressor factor activity, RANTES, and macrophage inflammato ry proteins 1 alpha and 1 beta. Allo-immunization also downregulated the pr oportion of cells with CCR5 and CXCR4 receptors. We also found a dose-depen dent decrease in HIV infectivity of CD4+ cells in vitro after allo-immuniza tion with both primary and T-cell line adapted HIV-1. This study provides a rational basis for an alternative or complementary strategy of allo-immuni zation against HIV infection.