Nucleotide sequence of the porcine p53 cDNA, and the detection of recombinant porcine p53 expressed in vitro with a variety of anti-p53 antibodies

The cDNA of porcine p53 was cloned and sequenced by a reverse transcriptase polymerase chain reaction (RT-PCR) approach with primers based on regions of homology between all known p53 sequences. The p53 cDNA was found to be 8 7% conserved to human p53 and 86% conserved to bovine p53 at the nucleotide level. The porcine p53 sequence was inserted into an expression vector and recombinant protein expressed in vitro. An approximately 50 kDa protein wa s detected by Western blotting using both polyclonal and monoclonal anti-p5 3 antibodies. The sequence data of porcine p53 and the ability to detect ex pressed protein with various anti-p53 antibodies will allow the p53 status of the pig population, and the role of p53 in porcine tumours, to be assess ed. An understanding of tumour development in the pig may be important if p ig cells, tissues or organs are to be used in the treatment of humans.