The ability to divide subsets of children with astrocytoma into prognostic
groups is limited because only a few clinical and pathologic variables are
available. This study evaluated DNA ploidy as a potential prognostic factor
in 30 children with diagnosed gliomas and examined the correlation of flow
cytometric analysis to other parameters such as sex, age at diagnosis, his
tologic grading, localization of tumor, and completeness of surgical resect
ion. Seventeen children, with low-grade glioma and 13 with high-grade gliom
a were retrospectively reviewed; mean age of the patients was 8.2 years, an
d mean follow-up of the population was 7.6 years. The tumor was localized t
o the cerebrum in 19 patients, the cerebellum in 7 patients, the brain stem
in 3 patients, and the spine in I patient. Fourteen patients underwent com
plete excision and 16 patients underwent partial excision. DNA diploidy was
demonstrated in 21 patients and aneuploidy in 9 patients. Twenty children
had no evidence of disease and 10 died of disease. Of the patients with dip
loid tumors, 81 % survived, compared to only 33% survival among patients wi
th aneuploid tumors (p < .011). By Cox regression analysis with age, gender
, type of excision, grade, location of tumor, and ploidy as independent var
iables, ploidy was a statistically significant predictor of survival (p = .
043). This investigation provides further evidence that flow cytometry may
have prognostic value in children with gliomas. Thus, a larger number of tu
mors can be studied to extend and validate these observations..