Increasing evidence suggests that microbial interactions are important dete
rminants of plant biodiversity. The hypothesis that fungal endophyte symbio
sis reduces diversity in successional fields was tested by manipulating inf
ection of tall fescue, the most abundant perennial grass in the eastern Uni
ted States. Over a 4-year period. species richness declined and tall fescue
dominance increased in infected plots relative to uninfected plots without
differences in total productivity. A host-specific endophyte, with negligi
ble biomass, altered plant community structure in this Long-term field expe
riment and may be reducing plant diversity throughout its expanding range.