Sulphuryl fluoride fumigation: effect on DNA extraction and amplification from herbarium specimens

Authors
Whitten, WM Williams, NH Glover, KV
Citation
Wm. Whitten et al., Sulphuryl fluoride fumigation: effect on DNA extraction and amplification from herbarium specimens, TAXON, 48(3), 1999, pp. 507-510
Citations number
6
Categorie Soggetti
Plant Sciences
Journal title
TAXON
ISSN journal
00400262 → ACNP
Volume
48
Issue
3
Year of publication
1999
Pages
507 - 510
Database
ISI
SICI code
0040-0262(199908)48:3<507:SFFEOD>2.0.ZU;2-J
Abstract
Dried leaf samples were exposed to sulphuryl fluoride (Vikane) during herba rium fumigation. DNA was extracted from fumigated leaf samples and from unf umigated controls. Both fumigated and control samples yielded good quality DNA; DNA amplification yielded similar products from both sets of samples. Vikane does not appear to degrade DNA present in herbarium specimens, and a ppears to be a non-destructive fumigant for pest control in herbaria.