The effect of egg yolk, low density lipoproteins, methylxanthines and fertilization diluent on cryopreservation efficiency of northern pike (Esox lucius) spermatozoa
I. Babiak et al., The effect of egg yolk, low density lipoproteins, methylxanthines and fertilization diluent on cryopreservation efficiency of northern pike (Esox lucius) spermatozoa, THERIOGENOL, 52(3), 1999, pp. 473-479
The-effect of egg yolk, low density lipoproteins (LDL) as well as methylxan
thines (caffeine and theophylline) and fertilization diluent on cryopreserv
ation efficiency of northern pike, Esox lucius, spermatozoa was tested, Mil
twas cryopreserved in pellets on dry ice then stored in liquid nitrogen. Th
e extender consisted of 0.6 M sucrose + 15% DMSO supplemented with egg yolk
or LDL fractions. The most effective results (77.3% hatched larvae vs 74.1
% in the control group) were obtained from extender that contained only 0.6
M sucrose + 15% DMSO and was used for freezing, while the fertilization di
luent was used for thawing. Addition of egg yolk or LDL to the extender did
not improve the results. The presence of caffeine in the thawing solution
significantly lowered fertilization rate of cryopreserved spermatozoa, wher
eas theophylline did not significantly affect the results. The addition of
fertilization diluent to the eggs prior to insemination was superior to the
other treatments. The proposed procedure constitutes a complete method for
the efficient cryopreservation of northern pike semen. (C) 1999 by Elsevie
r Science inc.