Regression of the hyaloid vessels and pupillary membrane of the mouse

Regression of the pupillary membrane (PM) and hyaloid vessels - hyaloid art eries (HAs), tunica vasculosa lentis (TVL), and vasa hyaloidea propria (VHP ) - in mice aged from 0 to 16 days was observed using stereomicroscopy and transmission electron microscopy. Whole-mount stereomicroscopy revealed tha t the pattern of normal developed vessels was basically the same as that re ported in rats and rabbits and that the VHP and PM disappeared between 12 a nd 16 days and 10 and 12 days, respectively, while certain examples of the TVL and HA remained even at 16 days. In the TVL, VHP and PM, regression occ urred segmentally and resulted in a decreased number of interconnections. T he ultrastructure of the vessels in the VHP, TVL and PM was consistent with a typical capillary with pericyte covering and no fenestrations. HAs had t unica media and adventitia in the older stages. Some endothelial cells in t he TVL and PM attaching to the lens capsule were thin at the side of the le ns. Many macrophages were observed in the vitreous and around vessels in th e whole-mount specimens at all stages. Some macrophages remained linearly a rranged even after vessels became vestigial and disappeared. In transmissio n electron microscopy, most of these macrophages were seen to possess vacuo les and/or processes, and some of them had phagosomes. Electron microscopic findings from regressing ocular vessels were consistent with the apoptosis of both endothelia and pericytes. Obstruction of the vessels was noted at older stages. These results add further anatomical information to previous studies and suggest that the VHP and TVL as well as PM regress via apoptosi s. The precise mechanisms of regression of hyaloid vessels and the role of macrophages remain for further studies.