Ev. Shumilina et al., Influence of coprecipitation and interactions with anionic surfactants on the activity of plant phospholipase D, BIOCHEM-MOS, 64(8), 1999, pp. 883-889
The effects of two surfactants (sodium dodecyl: sulfate and sodium dodecyl
dioxyethylene sulfate) of similar structure but differing water solubility
(of their calcium salts) on the enzymatic activity of cabbage phospholipase
D have been studied. The solubility difference is insignificant because th
e two surfactants activate phospholipase D similarly. To elucidate the mech
anism of their influence on the enzyme, the phase behavior in the:reaction
media and the interactions of the surfactants with the enzyme were investig
ated by potentiometry and by light scattering and UV spectroscopy. Calcium
dodecyl dioxyethylene sulfate (which is more soluble in water than calcium
dodecyl sulfate) precipitates in the: presence of phosphatidylcholine, the
substrate of the enzymatic reaction. In the reaction media phospholipase D
was involved into a precipitate consisting of calcium salts of the surfacta
nts and phosphatidylcholine that might be interpreted as its immobilization
. In addition, the surfactants were adsorbed on the enzyme, unfolding the g
lobular enzyme molecule due to electrostatic repulsion between adsorbed sur
factant anions. The observed increase in the functional activity of phospho
lipase D is accounted for by transfer to an optimal tertiary structure for
the enzyme molecule in the course of consecutive conformational transitions
induced by the surfactants.