The ABC-exporter genes involved in the lipase secretion are clustered withthe genes for lipase, alkaline protease, and serine protease homologues inPseudomonas fluorescens no. 33

In Pseudomonas fluorescens no. 33, the lipase gene is clustered with the ge nes for alkaline protease, AprDEF exporter, and two homologue proteins of S erratia serine proteases (pspA and pspB). Secretion of the lipase and alkal ine protease through AprDEF was shown in the Escherichia coli cells. Intere stingly, the E. coli cells carrying the pspA gene secreted PspA to the medi a AprDEF-independently. (C) 1999 Elsevier Science B.V. All rights reserved.