Cloning and expression of an Oerskovia xanthineolytica beta-1,3-glucanase in Escherichia coli

Authors
Salazar, O Molitor, J Asenjo, JA
Citation
O. Salazar et al., Cloning and expression of an Oerskovia xanthineolytica beta-1,3-glucanase in Escherichia coli, BIOTECH LET, 21(9), 1999, pp. 797-802
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
9
Year of publication
1999
Pages
797 - 802
Database
ISI
SICI code
0141-5492(199909)21:9<797:CAEOAO>2.0.ZU;2-J
Abstract
An Escherichia coli recombinant system produced a soluble beta-1,3-glucanas e (BglII) cloned from Oerskovia xanthineolytica. The protein was obtained i n a truncated form derived from the complete polypeptide. Cell fractionatio n studies show that 80% of the glucanase was retained in the periplasmic sp ace after 20 h of induction. If cells were grown with glycine, 60% of the g lucanase was released from the periplasm.