Kinetic study of the cellobiase activity of Trichoderma reesei cellulase complex at high substrate concentrations

Authors
Cascalheira, JF Queiroz, JA
Citation
Jf. Cascalheira et Ja. Queiroz, Kinetic study of the cellobiase activity of Trichoderma reesei cellulase complex at high substrate concentrations, BIOTECH LET, 21(8), 1999, pp. 651-655
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
8
Year of publication
1999
Pages
651 - 655
Database
ISI
SICI code
0141-5492(199908)21:8<651:KSOTCA>2.0.ZU;2-W
Abstract
At high cellobiose concentrations, the cellobiase activity of a Trichoderma reesei cellulase preparation does not follow Michaelis-Menten kinetics and shows substrate inhibition. Several rate equations were fitted to the init ial rate-cellobiose concentration data. The best fit is obtained for a rate equation corresponding to partial substrate inhibition of cellobiase. In t his case, the K-m, V(m)ax and K-I values obtained are 1.1 mM, 16 IU ml(-1) and 26 mM, respectively.