A high-density differential screening strategy for identification of foveagenes with altered expression in the retina during aging

A human fovea cDNA library was arrayed at high-density and reacted with age -specific (4 yr & 80 yr) retinal probes to identify genes with altered expr ession during aging. Using this approach, we screened 55,368 genes by South ern analysis and determined that similar to 0.7% are differentially express ed in young and old tissues. Northern analysis of total RNA from retina of humans aged 2-94 yr show that expression of one of the clones identified (c lone dd(112)) decreases with aging.