A rapid selection/amplification procedure for high-level expression of recombinant protein in a metal-amplifiable mammalian expression system

Authors
Bailey, CG Baig, M Gray, PP Sunstrom, NA
Citation
Cg. Bailey et al., A rapid selection/amplification procedure for high-level expression of recombinant protein in a metal-amplifiable mammalian expression system, BIOTECH TEC, 13(9), 1999, pp. 615-619
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
9
Year of publication
1999
Pages
615 - 619
Database
ISI
SICI code
0951-208X(199909)13:9<615:ARSPFH>2.0.ZU;2-V
Abstract
We describe a strategy for the selection and amplification of foreign gene expression in Chinese hamster ovary (CHO) cells employing a metallothionein gene-containing expression vector. This report describes an amplification procedure that results in an enrichment of clones exhibiting high levels of recombinant protein production and reduces the labour required for screeni ng recombinant cell lines.