Analysis of the pattern of gene expression during human adipogenesis by DNA microarray

High density DNA microarrays containing over 5000 cDNA clones were used to carry out a comprehensive investigation of gene expression during adipogene sis. Complex probes synthesized from total RNA were hybridized to the array s to determine the level of mRNA expression of each arrayed gene. Thirty th ree genes (29 known and 4 ESTs with no identified homologies) have been fou nd to alter their level of expression more than 2.5-fold after differentiat ion. The quantitative measurement by DNA array was in good agreement with c onventional Northern blot analysis of selected genes. Our results demonstra te that utilization of a DNA array is a speedy, efficient and quantitative approach to profile the expression of a large number of genes.