Oscillatory luminescence from lux-gene engineered bacteria

Authors
Simkus, R Csoregi, E Leth, S Corbisier, P Diels, L Mattiasson, B
Citation
R. Simkus et al., Oscillatory luminescence from lux-gene engineered bacteria, BIOTECH TEC, 13(8), 1999, pp. 529-532
Citations number
8
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
8
Year of publication
1999
Pages
529 - 532
Database
ISI
SICI code
0951-208X(199908)13:8<529:OLFLEB>2.0.ZU;2-3
Abstract
The experimental conditions for oscillatoryluminescence from Alcaligenes eu trophus harbouring luxCDABE genes inducible by copper were developed. The i ncubation of optimised bacterial suspensions (concentration of copper sulph ate - 2 mM, concentration of biomass - 0.15-0.20 mg ml(-1)) for 4-6 h under weak visible light followed by sudden transition of the cells to the dark resulted in pronounced oscillations with an average period of similar to 10 min.