Selection of capsule deficient strains of Methylobacterium rhodesianum producing polyhydroxybutyrate

Authors
Bormann, EJ Roth, M Linss, W Leissner, M
Citation
Ej. Bormann et al., Selection of capsule deficient strains of Methylobacterium rhodesianum producing polyhydroxybutyrate, BIOTECH TEC, 13(8), 1999, pp. 539-544
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
8
Year of publication
1999
Pages
539 - 544
Database
ISI
SICI code
0951-208X(199908)13:8<539:SOCDSO>2.0.ZU;2-Z
Abstract
Methylobacterium rhodesianum was cultivated using an extended fed-batch reg ime. After treatments with N-nitroso-methyl-urea or X-rays, colonies showin g an irregular morphology or increased heavy-metal sensitivity were selecte d. Electron micrographs of cells of isolated strains demonstrated the loss of the glycocalyx. Selectants and the parental strain yielded identical PHB amounts.