Pf. Li et al., Requirement for protein kinase C in reactive oxygen species-induced apoptosis of vascular smooth muscle cells, CIRCULATION, 100(9), 1999, pp. 967-973
Citations number
30
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Background-Vascular smooth muscle cell (VSMC) apoptosis is a component of a
variety of cardiovascular diseases and may be related to reactive oxygen s
pecies (ROS). This study was designed to deter mine the role of protein kin
ase C (PKC) in ROS-induced VSMC apoptosis,
Methods and Results-Rat aortic VSMCs were exposed to H2O2, and the nature o
f cell death was characterized in the absence or presence of different PKC
inhibitors. The results demonstrate that exposure of VSMCs to H2O2, led to
a dose-dependent (25 to 100 mu mol/L) and time-dependent (peal, at 2 minute
s) activation of PKC. Among the PKC isoforms alpha, beta, delta, epsilon, a
nd zeta, only PKC-alpha and PKC-epsilon were found to change their intracel
lular distribution on H2O2 treatment. Apoptosis was the predominant form of
cell death when PKC had been activated by H2O2 alone or by H2O2 in the pre
sence of 50 nmol/L phorbol 12-myristate 13-acetate. In contrast, necrosis b
ecame the predominant form of cell death when PKC had been downregulated by
prolonged exposure to 200 nmol/L phorbol 12,13-dibutyrate or inhibited by
50 nmol/L staurosporine, 100 nmol/L calphostin C, or 30 mu mol/L H-7, In ad
dition, caspase-3 was activated in H2O2-induced VSMC apoptosis but not when
PKC was downregulated or inhibited, Inhibition of caspase-3 by 50 mu mol/L
Ac-DEVD-CHO could significantly attenuate H2O2-induced apoptosis and was n
ot associated with the induction of necrosis.
Conclusions-We conclude that in VSMCs, PKC converts the ROS-induced signals
from necrotic cell death to the activation of an apoptotic cell death prog
ram. These data imply a novel and important role of PKC for the pathogenesi
s of such vascular diseases as atherosclerosis, restenosis, and hypertensio
n.