Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant

Authors
Baret, P Cesari, M Queiroz, C Rouch, C Meunier, JC Cadet, F
Citation
P. Baret et al., Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant, CR AC S III, 322(1), 1999, pp. 29-34
Citations number
15
Categorie Soggetti
Multidisciplinary,"Experimental Biology
Journal title
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES
ISSN journal
07644469 → ACNP
Volume
322
Issue
1
Year of publication
1999
Pages
29 - 34
Database
ISI
SICI code
0764-4469(199901)322:1<29:PACOAM>2.0.ZU;2-W
Abstract
We have discovered a multienzymatic complex in fresh young sugarcane leaves . Th is complex is constituted of three enzymes: PEPcase, NADP-MDH and mali c enzyme. After successive molecular sieving chromatography, we have obtain ed a highly purified sample of the complex which has a molecular weight of 711 kDa. Its functional interest has been evaluated by comparing the kineti c properties of the enzymes in their free forms to those in their complexed form. We show that the association of the three enzymes leads to important changes in their respective kinetic properties. ((C) Academie des sciences / Elsevier, Paris.).