Schild's equation and the best estimate of pA(2) value and dissociation constant of an antagonist

Citation
Sm. Jankovic et al., Schild's equation and the best estimate of pA(2) value and dissociation constant of an antagonist, CROAT MED J, 40(1), 1999, pp. 67-70
Citations number
11
Categorie Soggetti
General & Internal Medicine
Journal title
CROATIAN MEDICAL JOURNAL
ISSN journal
03539504 → ACNP
Volume
40
Issue
1
Year of publication
1999
Pages
67 - 70
Database
ISI
SICI code
0353-9504(199903)40:1<67:SEATBE>2.0.ZU;2-#
Abstract
Calculation of the pA2 value and dissociation constants for an antagonist f rom the effects observed on isolated smooth muscles can be done in two ways : using Schild's plot procedure or Schild's equation. In our study we used the effects of muscarinic antagonists observed in experiments on isolated h uman and feline stomach and rat gastric fundus. Only the estimates of pA2 v alues and dissociation constants made using the Schild's equation on the ba sis of the lowest antagonist concentrations were not significantly differen t from the values calculated using the Schild's plot procedure. This sugges t that, when it is impractical to perform the full Schild's plot procedure, the best estimate of pA2 values and dissociation constants can be done wit h the lowest antagonist concentration that significantly inhibits the effec ts of an agonist on an isolated smooth muscle preparation.