Regulation of epidermal growth factor receptor traffic by the small GTPaseRhoB

Citation
A. Gampel et al., Regulation of epidermal growth factor receptor traffic by the small GTPaseRhoB, CURR BIOL, 9(17), 1999, pp. 955-958
Citations number
16
Categorie Soggetti
Experimental Biology
Journal title
CURRENT BIOLOGY
ISSN journal
09609822 → ACNP
Volume
9
Issue
17
Year of publication
1999
Pages
955 - 958
Database
ISI
SICI code
0960-9822(19990909)9:17<955:ROEGFR>2.0.ZU;2-R
Abstract
Members of the Rho family of small GTPases control cell adhesion and motili ty through dynamic regulation of the actin cytoskeleton. Although twelve fa mily members have been identified, only three of these RhoA, Pac and Cdc42 - have been studied in detail. RhoA regulates the formation of focal adhesi ons and the bundling of actin filaments into stress fibres. It is also invo lved in other cell signalling pathways including the regulation of gene exp ression and the generation of lipid second messengers [1,2]. RhoA is very c losely related to two other small GTPases about which much less is known: R hoB and RhoC (which are approximately 83% identical). Perhaps the most intr iguing of these is RhoB. RhoA is largely cytosolic but translocates to the plasma membrane on activation. RhoB, however, is entirely localised to the cytosolic face of endocytic vesicles [3,4], This suggests a potential role for RhoB in regulating endocytic traffic; however, no evidence has been pre sented to support this. RhoA has been shown to act at the plasma membrane t o regulate the clathrin mediated internalisation of transferrin receptor [5 ] and of the muscarinic acetylcholine receptor [6]. We have recently demons trated that RhoB binds the RhoA effector, PRK1 and targets it to the endoso mal compartment [7]. We show here that RhoB acts through PRK1 to regulate t he kinetics of epidermal growth factor receptor traffic.