Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody

Authors
Putalun, W Tanaka, H Shoyama, Y
Citation
W. Putalun et al., Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody, CYTOTECHNOL, 31(1), 1999, pp. 151-156
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
CYTOTECHNOLOGY
ISSN journal
09209069 → ACNP
Volume
31
Issue
1
Year of publication
1999
Pages
151 - 156
Database
ISI
SICI code
0920-9069(1999)31:1<151:RSOSGB>2.0.ZU;2-T
Abstract
Immunoaffinity column using anti-solamargine monoclonal antibody for separa tion of solasodine glycosides was established. This method was specific for solasodine glycosides which was detected by thin layer chromatography and the western blotting. Total solasodine glycosides have been separated direc tly from the crude extract of Solanum khasianum fruit by the newly establis hed immunoaffinity column.