Variation in forest canopy structure influences both understory light avail
ability and its spatial distribution. Because light is a major environmenta
l factor limiting growth and survival of many forest species, its distribut
ion may affect stand-level regeneration patterns. We examined spatial patte
rning in light availability and seedling regeneration in old-growth, second
-growth, and selectively logged stands of tropical moist forest in northeas
tern Costa Rica. Our objectives were to determine how the frequency distrib
ution and spatial pattern of understory light "microsites" differ among tro
pical wet forests; whether patterns of seedling regeneration are linked to
spatial patterning of light availability; and whether these relationships d
iffer among old-growth, second-growth, and selectively logged forest stands
. We used both sensor-based and hemispherical photograph-based methods to m
easure light availability along three 130-160 m long transects in each of e
ight stands (three old-growth, three second-growth, and two selectively log
ged). Woody seedling abundance was assessed at 4 m(2), 25 m(2), and full-st
and scales (430 m(2)), and species richness was computed at the 25-m(2) and
full-stand levels. Data were analyzed using both conventional parametric a
pproaches and spatial statistics. Mean light availability did not differ ma
rkedly among stand types, but variance and frequency distributions of light
availability did. Second-growth stands had significantly higher unweighted
canopy openness along solar tracks and a higher frequency of microsites at
intermediate light levels. Old-growth stands had greater representation of
both low- and high-light microsites, and greater overall variance in light
availability. Old-growth stands also had slightly higher abundance and spe
cies richness of woody seedlings. Light availability was significantly spat
ially autocorrelated in all stand types, but patch size (analogous to gap s
ize) was twice as large in old-growth stands, based on sensor data. Seedlin
g abundance was also spatially autocorrelated over greater distances in old
-growth than in second-growth stands, often at similar spatial scales to li
ght distribution. The selectively logged stands demonstrated spatial autoco
rrelation of light and seedling abundance over distances intermediate to th
e other two stand types. Despite the similarities in patterns of light and
seedling distributions, relationships between woody seedling abundance, spe
cies richness, and the three light availability measures were not strong or
consistently positive, regardless of whether standard regressions or parti
al Mantel tests were applied. Although seedling abundance is likely to be a
ffected by a wide variety of factors, the similarities in the scales of spa
tial autocorrelation of light and seedling abundance suggest that current s
eedling abundance distributions may reflect past patterns of light distribu
tion within the stands. Our results confirm the importance of examining spa
tial dependence of resource availability in studies of forest dynamics, but
they also underscore the limitations of a single period of data collection
. Long-term studies as well as experimental manipulations of resource avail
ability are needed to establish causal relationships between resource avail
ability and stand-level patterns of seedling regeneration.